Next Generation Sequencing (NGS) Services

Solicitation description

NEXT GENERATION SEQUENCING SOLICITATION # 68HERC24Q0155 THIS IS A SOURCES SOUGHT SYNOPSIS FOR WRITTEN INFORMATION ONLY. THIS IS NOT A SOLICITATION ANNOUNCEMENT FOR PROPOSALS AND NO CONTRACT WILL BE AWARDED FROM THIS ANNOUNCEMENT. The Environmental Protection Agency (EPA) is performing a "market search" for small businesses in preparation for a future procurement. To help determine if this potential requirement may be set-aside for small business concerns, under the range of potential acquisition approaches the government may pursue, the government is seeking input from the small business community regarding their interest and capability in performing the tasks reflected in the attached draft Performance Work Statement (PWS) as well as their availability to perform under various strategic sourcing solutions. Information submitted in response to this notice is merely used for internal market research purposes, and no feedback will be offered to the interested parties that submit capability statements. To broaden the Agency’s market research effort EPA will also utilize GSA’s Market Research as a Service (MRAS) entities may respond to either, as the information collected from both outreach notices will be used to help determine if this potential requirement may be set-aside for small business concerns, under the range of potential acquisition approaches the government may pursue. The United States Environmental Protection Agency will be using next generation sequencing (NGS) to complete current and future gene expression translational research to fulfill obligations to SSWR.3, CSS.4.4, CSS.6.4, and CSS.4.3 research programs. Historically, the USEPA has used microarray and quantitative polymerase chain reaction technologies to perform these studies. However, recent advances in technology, coupled with significant decreases in supply costs, have made sequencing-based gene expression studies more informative and cost-effective. Market data suggest that this trend should continue or even improve for the foreseeable future, thereby facilitating the change to NGS. Specific areas to be addressed in capability packages submitted by respondents are as follows: Capability 1: Ability to accept user-prepared sequencing libraries. Capability 2: Access to short-read DNA and RNA sequencing with a capacity equal to or greater than NovaSeq6000 S4 flow cell Capability 3: Ability to prepare DNA and RNA sequencing libraries compatible with Illumina instrumentation at a capacity of 10000 – 3000 samples per year. Capability 4: Capacity to perform sequencing on 10000 – 3000 samples per year. Capability 5: Ability to prepare 3’-tagged RNA-seq libraries, such as QuantSeq. Capability 6: Capability to prepare libraries in and perform full length RNA sequencing, such as PacBio or Oxford Nanopore. Capability 7: Ability to perform QA/QC analysis, such as Qubit and TapeStation, for in-house and user submitted sequencing library preparations. Capability 8: Ability to upload...

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